Fenótipos de resistência à meticilina, macrolídeos e lincosamidas em Staphylococcus aureus isolados de um hospital de Valledupar, Colombia

Gloria Inés Morales Parra; María Cecilia Yaneth Giovanetti; Andrés Zuleta Hernández

doi:10.12804/revsalud14.02.2016.07

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Phenotype Resistance to Methicillin, Macrolides and Lincosamides in Staphylococcus Aureus, Isolated in a Hospital in Valledupar, Colombia

DOI: https://doi.org/10.12804/revsalud14.02.2016.07

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Published: May 31, 2016

Issue: Vol. 14 No. 02 (2016)

Section Clinical or Experimental Research

Keywords: resistencia bacteriana meticilina macrólidos lincosamidas MLSBc MLSBi.

Authors

Gloria Inés Morales Parra
Universidad de Santander.

María Cecilia Yaneth Giovanetti
Universidad de Santander.

Andrés Zuleta Hernández
Universidad de Santander.

Abstract

Introduction: Infections with methicillin-resistant S. aureus are a public health problem due to the multi-resistance profile presented by this pathogen. Objective: To determine resistance phenotypes to methicillin, macrolides and lincosamides in S. aureus. Materials and methods: 50 S. aureus strains, isolated from patients of the Hospital Rosario Lopez Pumarejo in the city of Valledupar, were analyzed. Susceptibility tests to methicillin, erythromycin and clindamycin were performed using microdilution and agar diffusion methods. Methicillin resistance was determined through agar dilution technique and inducible clindamycin resistance D-Test. Results: Methicillin resistance reached 50 %, five phenotypes were established in the analyzed macrolides and lincosamides: phenotype sensitive to erythromycin and clindamycin (78 %); phenotype resistant to erythromycin and clindamycin (16 %) with constitutive resistance for both cMLSB antimicrobials, which lead the resistance phenotypes; phenotype with intermediate resistance to both antimicrobials (2 %); the intermediate result phenotype resistant to erythromycin and clindamycin (2 %); and the RS phenotype resistant to erythromycin and sensitive to clindamycin (2 %) that show inducible iMLSB clindamycin resistance with positive D test. Conclusions: The inducible resistance to macrolides, lincosamides and streptogramines is not established through the standard antimicrobial susceptibility test. Not identifying the inducible resistance can lead to clindamycin treatment failure.

Author Biographies

Gloria Inés Morales Parra, Universidad de Santander.

MsC. Universidad de Santander.

María Cecilia Yaneth Giovanetti, Universidad de Santander.

MsC. Universidad de Santander.

Andrés Zuleta Hernández, Universidad de Santander.

Universidad de Santander.

How to Cite

Morales Parra, G. I., Giovanetti, M. C. Y., & Zuleta Hernández, A. (2016). Phenotype Resistance to Methicillin, Macrolides and Lincosamides in Staphylococcus Aureus, Isolated in a Hospital in Valledupar, Colombia. Revista Ciencias De La Salud, 14(02), 223–231. https://doi.org/10.12804/revsalud14.02.2016.07

References

Castañón-Sánchez CA. Patogenia molecular de Staphylococcus aureus. Evid Med Invest Salud. 2012; 5(3):79-84.

Centro para el Control y Prevención de Enfermedades. Antibiotic resistance threats in the United States, 2013.

Lall M, Sahni AK. Prevalence of inducible clindamycin resistance in Staphylococcus aureus isolated from clinical samples. Med J Armed Forces India. 2014; 70(1):43-47.

Tamariz-Ortiz JH, Cruz-Quintanilla J, Atencia-Porras A, Figueroa-Tataje J, Horna-Quintana G, Guer¬ra-Allison H, et al. Resistencia a clindamicina inducida por eritromicina en Staphylococcus aureus aislados de tres hospitales de Lima, Perú. Acta méd. Peruana. 2009; 26(1):12-16.

Clinical Laboratory Standardization Institute Performance Standards for antimicrobial susceptibility testing. Nineteenth Informational Supplement. CLSI Documnt M100-S23. Table 2C. Supplement table 1. 2013; 33(1):80-81.

Pérez N, Pavas N, Rodríguez EI. Resistencia de Staphylococcus aureus a los antibióticos en un hospital de la Orinoquía Colombiana. Infectio. 2010; 14(3):167-173.

Mariem JJ, Ito T, Zhang M, Jin J, Li S, Boutiba B, et al. Molecular characterization of methicillin-re¬sistant Panton-valentine leukocidin positive Staphylococcus aureus clones disseminating in Tunisian hospitals and in the community. Rev. BMC Microbiology. 2013; 13(2):2-8.

Guzmán-Lista M, Lozada-Oca RA. Detección de Staphylococcus aureus meticilino-resistentes aislados de pacientes con infecciones nosocomiales y adquiridas en la comunidad. Rev. Soc. Ven. Microbiol. 2007; 27(1):349-363.

Perazzi B, Camacho M, Bombicino K, Flores Z, Vay C, Famiglietti A. Staphylococcus aureus: nuevos y antiguos antimicrobianos. Rev. Argent. Microbiol. 2010; 42(3):199-202.

Merino-Díaz L, Cantos de la Casa A, Torres Torres-Sánchez M, Aznar J. Detección de resistencia indu-cible a clindamicina en aislados cutáneos de Staphylococcus spp. por métodos fenotípicos y genotípicos. Enferm Infecc Microbiol Clin. 2007; 25(2):77-81.

Baños-Rodríguez Á. Microorganismos multirresistentes, ¿adquisición nosocomial o comunitaria? Rev Enferm Infecc Microbiol Clin. 2004; 22(9):505-6.

Castellano-González MJ, Perozo-Mena AJ, Vivas-Vega RL, Ginestre-Pérez MM, Rincón-Villalobos GC. Tipificación molecular y fenotípica de Staphylococcus aureus resistentes a meticilina (samr) en un hospital universitario. Rev. Chil. Infectol. 2009; 26(1):39-48.

Almeida E, Da Costa A, Gir E, Pimenta F, Vanzato I. Prevalencia de Staphylococcus aureus na saliva de trabalhadores de saúde. Colomb Méd. 2011; 42(2):10-16.

Pérez-Tapia AG, Sánchez-Vázquez M, Bautista M, Mendosa-Charcas DC, Fragoso-Morales R, Velarde LE, et al. Prevalencia de infección de herida quirúrgica, causas y resistencia a los fármacos en el Hospital General de Zona núm. 2 del IMSS, San Luis Potosí. Rev Esp Méd Quir. 2012; 17(4):261-265.

Anodal M, Gardella N, Merola G, Mollerach M, Rodríguez L, Schijman M, et al. Infecciones de piel y partes blandas por Staphylococcus aureus meticilino resistente de la comunidad. Análisis molecular y genético. Rev. Dermatol. Argent. 2012; 18(3):213-220.

Ocampo AM, Vélez NA, Robledo J, Jiménez JN. Cambios a lo largo del tiempo en la distribución de los complejos de clones dominantes de Staphylococcus aureus resistente a la meticilina en Medellín, Colombia. Biomédica. 2013; 34(1):34-40.

Reyes J, Rincón S, Díaz L, Panesso D, Contreras GA, Zurita J, et al. Dissemination of Methicillin-Re¬sistant Staphylococcus aureus (mrsa), USA300 Sequence Type 8 Lineage in Latin-America. Clinical infectious diseases: an official publication of the Infectious JIDI. 2009; 49(12):1861-1867.

Prabhu K, Sunil R, Venkatakrishna R. Inducible Clindamycin Resistance in Staphylococcus aureus Isolated from Clinical Samples. J Lab Physicians. 2011; 3(1):25-27.

Vineeta M, Sachin K, Siddique ME. Prevalence of inducible clindamycin resistance among clinical isolates of Staphylococcus aureus detected by phenotypic method: A preliminary report. J Infect Dis Immun. 2013; Rev Esp Méd Quir 5(1):10-12.

Patil NR, Mali US, Kulkarni SA, Ghorpadeand MV, Mane V. Detection of inducible clindamycin resis¬tance among clinical isolates of Staphylococcus aureus in a tertiary care hospital. Int. J. Curr. Microbiol. App. Sci. 2014; 3(9)689-694.

Ghogare HS, Hatkar SS, Bansal MP. Phenotypic Detection of Inducible Clindamycin Resistance among the Clinical Isolates of Staphylococcus aureus by Using D-Test. IJHSR. 2014; 4(3):149-153.

Durmaz S, Kiraz A, Toka T, Perçin D. Macrolide-Lincosamide-Streptogramin B Resistance Phenotypes in Staphylococcus aureus. Eur J Gen Med. 2014; Diseases Society of America 11(4):217-220.

Motamedifar M, Ebrahim HS, Mansury D. Patterns of Constitutive and Inducible Clindamycin Resis-tance in Staphylococcus aureus Isolated from Clinical Samples by D-test Method, Shiraz, Southwest of Iran. GMJ. 2014; 3(4):216-21.

Sandrea-Toledo LB, Piña-Reyes EJ, Paz-Montes A, Torres-Urdaneta EL. Determinación de la resistencia a meticilina y eritromicina de cepas de Staphylococcus aureus aisladas en un hospital del estado Zulia. Rev Soc Ven Microbiol. 2012; 32(2):88-94.

Uzunović S, Ibrahimagić A, Kamberović F, Kunarac M, Michelle I, Rijnders A. Inducible clindamycin resistance in methicillin-susceptible and methicillin-resistant Staphylococcus aureus of inpatient, outpatient and healthy carriers in Bosnia and Herzegovina. Med Glas (Zenica) 2013; 10(2):217-224.

Juyal D, Shamanth A, Pal S, Sharma MK, Prakash R, Sharma N. The Prevalence of Inducible Clin-damycin Resistance Among Staphylococci in a Tertiary Care Hospital – A Study from the Garhwal Hills of Uttarakhand, India. J Clin Diagn Res. 2013; 7(1):61-5.

Montoya CI, Mira OM, Álvarez AI, Cofre GJ, Cohen VJ, Donoso WG et al. Resistencia inducible a clindamicina en Staphylococcus aureus meticilino resistente. Rev. Chil. Pediatr. 2009; 80(1):48-53.

Levin TP, Suh B, Axelrod P, Truant AL, Fekete T. Potential clindamycin resistance in clindamycin-susceptible, erythromycin-resistant Staphylococcus aureus: Report of a clinical failure. Antimicrob Agents Chemother. 2005; 49(3):1222-4.

Delialioglu N, Aslan G, Ozturk C, Baki V, Sen S, Emekdas G. Inducible clindamycin resistance in sta-phylococci isolated from clinical samples. Jpn J Infect Dis. 2005; 58:104-6.

O’Sullivan MBN, Cai Y, Kong F, Zeng X, Gilbert GL. Influence of Disk Separation Distance on Accuracy of the Disk Approximation Test for Detection of Inducible Clindamycin Resistance in Staphylococcus spp. J Clin Microbiol. 2006; 44(11):4072-4076.

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