Antibacterial Evaluation of Hydrogen Peroxide Compared to Sodium Hypochlorite on Dental Brushes Inoculated with Streptococcus Mutans

DOI:  https://doi.org/10.12804/revistas.urosario.edu.co/revsalud/a.10226

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Published: Feb 16, 2021
Issue: Vol. 19 No. 1 (2021): Ciencias de la Salud
Section Clinical or Experimental Research


Keywords: Disinfection hydrogen peroxide sodium hypochlorite Streptococcus mutans

Main Article Content

Authors

César Félix Cayo-Rojas
Universidad Privada San Juan Bautista; Universidad Nacional Federico Villarreal
Estefany Hilda Rojas-Zubizarreta
Universidad Nacional Federico Villarreal
Miriam Kelly Nicho-Valladares
Universidad Nacional Federico Villarreal
Marysela Irene Ladera-Castañeda
Universidad Nacional Federico Villarreal; Universidad Privada San Juan Bautista
Ana Sixtina Aliaga-Mariñas
Universidad Nacional Federico Villarreal

Abstract

Objective: To assess the antibacterial effect of 6% hydrogen peroxide (H2O2) compared with 1% and 2% sodium hypochlorite, on toothbrushes inoculated with Streptococcus mutans ATCC® 25175™. Materials and methods: The study design was an experimental in vitro, cross-sectional prospective and comparative study. Sixty toothbrushes were used, which were divided into four groups of 15 brushes. After conducting a pilot study, the sample number was determined by the means comparison formula and these were selected by simple random sampling. These brushes were inoculated with strains of S. mutans ATCC® 25175™. The disinfectants included H2O2 at 6% and sodium hypochlorite (NaOCl) at 1% and 2%. The Shapiro–Wilk test was used to assess normality and the Mann-Whitney U test was applied for the hypothesis test. Results: The 6% H2O2 showed an average growth of 2 × 109 CFU/mL, which indicates that its effectiveness is greater compared to the 1% NaClO that showed a growth average of 4 × 109 CFU/mL which is less than the 2% NaClO that presented 0 CFU/mL of S. mutans (p = 0.004). Conclusion: Between 6% H2O2 and 1% and 2% NaClO, specifically antibacterial detection, 2% NaClO was concluded as being the most effective.

How to Cite
Cayo-Rojas, C. F., Rojas-Zubizarreta, E. H., Nicho-Valladares, M. K. ., Ladera-Castañeda, M. I. ., & Aliaga-Mariñas, A. S. (2021). Antibacterial Evaluation of Hydrogen Peroxide Compared to Sodium Hypochlorite on Dental Brushes Inoculated with Streptococcus Mutans. Revista Ciencias De La Salud, 19(1). https://doi.org/10.12804/revistas.urosario.edu.co/revsalud/a.10226
References

Villacis Sánchez JL. Identificacion de Enterococcus faecalis en cepillos dentales y evaluación in vitro de su grado de susceptibilidad frente a hipoclorito de sodio y gluconato de clorhexidina [tesis de grado en internet]. Quito: Universidad de las Américas; 2016. Disponible en: http://dspace.udla.edu.ec/handle/33000/5528

Salazar-Chicaiza SA, Zurita-Solis MK. Presencia de microorganismos en cepillos dentales y su desinfección con H2O2. Dom Cien [internet]. 2016;2(1):155-67. Disponible en: https:// docplayer.es/27162535-Presencia-de-microorganismos-en-cepillos-dentales-y-su-desin- feccion-con-h2o2-presence-of-microorganisms-on-toothbrushes-and-disinfection-with- h-2-o-2.html

Abranches J, Zeng L, Kajfasz JK, Palmer SR, Chakraborty B, Wen ZT, et al. Biology of oral Streptococci. Microbiol Spectr. 2018;6(5):1-18. https://doi.org/10.1128/microbiolspec. GPP3-0042-2018

Gevers D, Knight R, Petrosino JF, Huang K, McGuire AL, Birren BW, et al. The Human Microbiome Project: a community resource for the healthy human microbiome. PLoS Biol. 2012;10(8):e1001377. https://doi.org/10.1371/journal.pbio.1001377

Huse SM, Ye Y, Zhou Y, Fodor AA. A core human microbiome as viewed through 16S rRNA sequence clusters. PLoS One. 2012;7(6):e34242. https://doi.org/10.1371/journal. pone.0034242

Nascimento MM, Zaura E, Mira A, Takahashi N, Ten Cate JM. Second era of OMICS in caries research: moving past the phase of disillusionment. J Dent Res. 2017;96(7):733-40. https://doi.org/10.1177 / 0022034517701902

Do Nascimento C, Balero M, Murillo SP, Carriço FH, Linares P, Watanabeet E, et al. Effectiveness of three antimicrobial mouthrinses on the disinfection of toothbrushes stored in closed containers: a randomized clinical investigation by DNA checkerboard and culture. Gerondontology. 2014;31(3):227-36. https://doi.org/10.1111/ger.12035

Frazelle MR, Munro CL. Toothbrush contamination: a review of the literature. Nurs Res Pract. 2012;2012: 420630. https://doi.org/10.1155/2012/420630

Assed Bezerra Da Silva L, Nelson-Filho P, Saravia ME, De Rossi A, Lucisano MP, Assed Bezerra Da Silva R. Mutans streptococci remained viable on toothbrush bristles, in vivo, for 44 h. Int J Paediatr Dent. 2014;24(5):367-72. https://doi.org/10.1111/ipd.12079

Basman A, Peker I, Akca G, Alkurt MT, Sarikir C, Celik I. Evaluation of toothbrush disinfection via different methods. Braz Oral Res. 2016;30:S1806-83242016000100203. https:// doi.org/10.1590/1807-3107BOR-2016.vol30.0006

Akca E, Akca G, Toksoy F, Macit N, Pikdöken L, Özgen Y. The comparative evaluation of the antimicrobial effect of propolis with chlorhexidine against oral pathogens: an in vitro study. Biomed Res Int. 2016;2016:3627463. https://doi.org/10.1155/2016/3627463

Peker I, Akca G, Sarikir C, Toraman M, Celik I. Effectiveness of alternative methods for toothbrush disinfection: an in vitro study. ScientificWorldJournal. 2014;2014:726190. https://doi.org/10.1155/2014/726190

Salvia AC, Matilde FS, Rosa FC, Kimpara ET, Jorge AO, Balducci I, et al. Disinfection protocols to prevent cross contamination between dental offices and prosthetic laboratories. J Infect Public Health. 2013;6(5):377-82. https://doi.org/10.1016/j.jiph.2013.04.011

Sánchez Ruiz FH, Furuya Meguro AT, Arroniz Padilla S, Gómez Moreno A, Gómez L. Comparación de la acción bactericida del hipoclorito de sodio y Microcyn 60. Rev Odont Mex. 2009. 13(1):9-16. https://doi.org/10.22201/fo.1870199xp.2009.13.1.15613

Muratovska I, Kitagawa H, Hirose N, Kitagawa R, Imazato S. Antibacterial activity and dentin bonding ability of combined use of Clearfil SE Protect and sodium hypochlorite. Dent Mater J. 2018;37(3):460-64. https://doi.org/10.4012/dmj.2017-294

Lamont RJ, Hajisshengallis GN, Jenkinson HF. Microbiología e inmunología oral. México: Manual Moderno; 2015.

Ahn KB, Kim AR, Kee-Yeon K, Cheol-Heui Y, Seung H. The synthetic human beta-defen-sin-3 C15 peptide exhibits antimicrobial activity against Streptococcus mutans, both alone and in combination with dental disinfectants. J Microbiol. 2017;55(10):830-36. https://doi.org/10.1007/s12275-017-7362-y

Dadpe MV, Dhore SV, Dahake PT, Kale YJ, Kendre SB, Siddiqui AG. Evaluation of antimicrobial efficacy of Trachyspermum ammi (Ajwain) oil and chlorhexidine against oral bacteria: an in vitro study. J Indian Soc Pedod Prev Dent. 2018;36(4):357-63. https://doi. org/10.4103/JISPPD.JISPPD_65_18

Han-Sol C, Dong-Hoon S. Antibacterial capacity of cavity disinfectants against Streptococcus mutans and their effects on shear bond strength of a self-etch adhesive. Dent Mater J. 2016;35(1):147-52. https://doi.org/10.4012/dmj.2015-175

Hernández Sampieri R, Fernández Collado C, Baptista Lucio P. Metodología de la investigación. 6.a ed. México: McGraw-Hill; 2014.

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