Comparison and Evaluation of Quantitative Methods for Determining Antimicrobial Susceptibility of Complex Mycobacterium Abscessus Strains

DOI:  https://doi.org/10.12804/revistas.urosario.edu.co/revsalud/a.6491

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Published: Jan 31, 2018
Issue: Vol. 16 No. 1 (2018): Ciencias de la Salud
Section Clinical or Experimental Research


Keywords: antimicrobial susceptibility colorimetric micromethod broth microdilution minimum inhibitory concentration.

Main Article Content

Authors

Ana Ramírez
Laboratorio de Microbiología Molecular, Departamento de Microbiología y Parasitología, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida.
Nora Morcillo
Hospital Dr. Antonio A. Cetrángolo, Laboratorio de Referencia del Programa de Control de la Tuberculosis de la Provincia de Buenos Aires.
Belén Imperiale
Instituto Nacional de Tecnología Agropecuaria, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto de Biotecnología, Castelar, Buenos Aires.
María Araque
Laboratorio de Microbiología Molecular, Departamento de Microbiología y Parasitología, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida.
Jacobus H. De Waard
Universidad Central de Venezuela Laboratorio de Tuberculosis. Instituto de Biomedicina, San José, Caracas.

Abstract

Introduction: The Mycobacterium abscessus complex includes multidrug resistant emerging pathogens, which limit therapeutic options for treating infections caused by these microorganisms. In this study, the minimum inhibitory concentrations —mics— obtained by 2 quantitative methods were compared, the cut-off points used in the colorimetric micromethod —cmm— were established and the antimicrobial susceptibility was evaluated. Materials and Methods: The mic for nine antibiotics was determined by cmm and broth microdilution —bmd— for 19 strains of M. abscessus complex. The Snedecor F test was used to establish the significant difference in the cim between the methods, cutoff points were determined by the probability distribution method for the cmm. Results and Discussion: A correlation of 50% between cmm and bmd for antibiotics tested was found. Probably, this discrepancy in the results is due to differences in some technical parameters of each procedure. All strains were susceptible to amikacin and were resistant to meropenem and ampicillin-sulbactam. Independently of the species of M. abscessus complex, fluoroquinolones showed a low inhibitory activity (0-25%) on clinical isolates, results that are similar to those reported by other authors. The Multidrug resistance patterns observed in the strains tested suggest the need for susceptibility testing as tools to guide and optimize the therapeutic behavior in infections caused by M. abscessus. 

How to Cite
Ramírez A., Morcillo, N., Imperiale, B., Araque, M., & De Waard, J. H. (2018). Comparison and Evaluation of Quantitative Methods for Determining Antimicrobial Susceptibility of Complex Mycobacterium Abscessus Strains. Revista Ciencias De La Salud, 16(1), 69–81. https://doi.org/10.12804/revistas.urosario.edu.co/revsalud/a.6491
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