Monitoring of the Viability of Platelet Concentrates Obtained from Buffy Coat during Seven Days of Storage

DOI:  https://doi.org/10.12804/revistas.urosario.edu.co/revsalud/a.10720

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Published: Sep 20, 2021
Issue: Vol. 19 No. 3 (2021): Ciencias de la Salud
Section Clinical or Experimental Research


Keywords: validation platelet concentrate buffy coat viability

Main Article Content

Authors

Clara E. Arroyo Rubio
Pontificia Universidad Católica del Ecuador
  Katherine E. Jaramillo Ruales
Pontificia Universidad Católica del Ecuador
  Rosa F. Chiriboga Ponce
Pontificia Universidad Católica del Ecuador

Abstract

Introduction: Platelet concentrates (CPQ) are labile blood components affected by several factors from the method of production to storage conditions that cause a gradual loss of functionality. For this reason, it is necessary to evaluate the platelet quality parameters that guarantee the viability during the storage days, with the purpose of monitoring the maintenance of the functional characteristics of the platelets. Materials and methods: This cross-sectional descriptive study had a sample size of 64 platelet concentrates, evaluated at 3, 5, and 7 days of storage. The monitored parameters were the physical storage parameters and percentage of platelet activation by measuring P-selectin (CD62) via flow cytometry. The chi-square statistic, one-way Anova, Kruskal–Wallis test, and Pearson correlation were applied. Results: Significant differences were observed on the 7th day in relation to the 3rd and 5th day of storage, especially in the swirling parameter (p < 0.005) and platelet aggregates (p = 0.001). The platelet activation increased significantly (p = 0.001) on the 5th day. Conclusions: Based on the findings of this study, the viability of the platelet concentrates differs with the days of storage. For this reason, it is necessary to evaluate the swirling, pH, and aggregates to all platelet concentrates before being transfused as an indication of platelet activation and decreased functionality.

How to Cite
Arroyo Rubio, C. E., Jaramillo Ruales , K. E., & Chiriboga Ponce, R. F. (2021). Monitoring of the Viability of Platelet Concentrates Obtained from Buffy Coat during Seven Days of Storage. Revista Ciencias De La Salud, 19(3), 1–13. https://doi.org/10.12804/revistas.urosario.edu.co/revsalud/a.10720
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