Assessment of Modulatory and Cytotoxic Activity of the Essential Oil in Hyptis Martiusii Benth Leaves

DOI:  https://doi.org/10.12804/revistas.urosario.edu.co/revsalud/a.6489

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Published: Jan 31, 2018
Issue: Vol. 16 No. 1 (2018): Ciencias de la Salud
Section Clinical or Experimental Research


Keywords: actividad antibacteriana , actividad citotóxica , cidreira do campo , Hyptis martiusii , aceites esenciales Antibacterial activity , cytotoxic activity , cidreira do campo , Hyptis martiusii , essential oil atividade antibacteriana , atividade citotóxica , cidreira do campo , Hyptis martiusii , óleo essencial

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Authors

Francisco Rodolpho Sobreira Dantas Nóbrega de Figueirêdo Departamento de Química Biológica, Universidade Regional do Cariri —Urca—.
Ana Jaqueline Bitu Primo Departamento de Química Biológica, Universidade Regional do Cariri —Urca—.
Álefe Brito Monteiro Departamento de Química Biológica, Universidade Regional do Cariri —Urca—.
Saulo Relison Tintino Departamento de Química Biológica, Universidade Regional do Cariri —Urca—.
Gyllyandeson De Araújo Delmondes Departamento de Química Biológica, Universidade Regional do Cariri —Urca—.
Valterlúcio Dos Santos Sales Departamento de Química Biológica, Universidade Regional do Cariri —Urca—.
Cristina Kelly De Souza Rodrigues Departamento de Química Biológica, Universidade Regional do Cariri —Urca—.
Cicero Francisco Bezerra Felipe Departamento de Biologia Molecular, Universidade Federal da Paraíba —UFPB—.
Henrique Douglas Melo Coutinho Departamento de Química Biológica, Universidade Regional do Cariri —Urca—.
Marta Regina Kerntopf Departamento de Química Biológica, Universidade Regional do Cariri —Urca—.
Abstract
The species Hyptis martiusii Benth. is popularly known as “cidreira do campo”. It has multiple biological properties, including antifungal, anti-inflammatory, and insecticide. The aim of this study was to investigate the modulating activity of the essential oil of fresh leaves of Hyptis martiusii in association with different antibiotics against the bacteria Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, as also the cytotoxic activity of this species. The study used the MIC (minimum inhibitory concentration) as a way of assessing the modulation using an inoculation of 100 microliters of each bacterial strain. Cytotoxicity tests were conducted using in vitro culture of mouse connective tissue cells (fibroblasts of mammals). The research demonstrated that the essential oil from leaves of Hyptis martiusii Benth —oehm— presented synergism only in association with the antibiotics gentamicin and imipenem against the bacteria Pseudomonas aeruginosa and Escherichia coli. However, it presented antagonism in association with amikacin, gentamicin and imipenem against the three studied bacteria. In addition to the cipro oxacin showed no relevant results. In relation to the cytotoxic activity, the lethal concentration (LC50) exhibited a value of 263.12 μg/ml. Finally, we can say that H. Martiusii presents a synergistic cytotoxic activity against the evaluated bacteria.
How to Cite
Sobreira Dantas Nóbrega de Figueirêdo F. R., Bitu Primo, A. J., Brito Monteiro, A., Relison Tintino, S., De Araújo Delmondes G., Dos Santos Sales, V., … Kerntopf, M. R. (2018). Assessment of Modulatory and Cytotoxic Activity of the Essential Oil in Hyptis Martiusii Benth Leaves. Revista Ciencias De La Salud, 16(1), 49–58. https://doi.org/10.12804/revistas.urosario.edu.co/revsalud/a.6489
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