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Introdução: o Maslach Burnout Inventory-Human Service Survey (MBI-HSS) é um instrumento de medição que foi aplicado em médicos peruanos utilizando o Inquérito Nacional de Satisfação de Usuários em Saúde (Ensusalud) no ano 2014. No entanto, a evidência de validade de sua estrutura interna não se tem elaborado até hoje e seu exame parece necessário devido às diferentes propostas que existem sobre sua configuração fatorial. O presente estudo tem por objetivo, examinar a validez da estrutura interna do MBI-HSS em médicos peruanos com dados secundários do inquérito Ensusalud. Materiais e métodos: a amostra esteve conformada por 2222 médicos de diferentes regiões do Peru selecionados a partir de amostragem probabilística bi-etápica e estratificado. A validade da estrutura interna do MBI-HSS foi avaliada através da análise fatorial exploratória e confirmatória; a confiabilidade foi estimada segundo a consistência interna (coeficientes alfa e ômega); para além, avaliou-se o funcionamento diferencial dos itens (DIF) segundo o sexo. Resultados: demostra-se uma estrutura interna de três fatores no MBI-HSS com redução de três itens; a confiabilidade foi adequada (entre 0.845 e 0.918), ainda que diminuíra consideravelmente em presença de erros correlacionados (entre 0.335 y 0.517); quanto ao DIF se encontrou que o item 10 apresentara variação segundo o sexo. Conclusão: a versão original (22 itens) do MBI-HSS não é pertinente para a avaliação do Burnout em médicos peruanos sendo viável a proposta de 19 itens, ainda que sendo um estudo inicial de validação se requererá uma réplica de resultados.

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